A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle

A multiplex polymerase chain reaction (PCR) test was developed for identifying gastrointestinal (GI) nematodes that commonly infect cattle. This assay was developed using adult-derived genomic DNA and shown capable of discriminating parasite eggs from the feces of experimentally-infected animals at both the species and genus levels. Sequence data from internal (ITS) and external (ETS) transcribed spacers of the ribosomal DNA (rDNA) repeats as well as the 3′-end of the small subunit rDNA and 5′-end of the large subunit rDNA were used to generate five primer sets which, when used simultaneously in a multiplex PCR, produce a unique electrophoretic DNA banding pattern charac- terized by a single DNA fragment for Ostertagia ostertagi (257 bp), Haemonchus placei (176 bp),Oesophagostomum radiatum (329 bp), Trichostrongylus colubriformis (243 bp) and Cooperia on- cophora (151 bp). In a similar manner, the constructed primer sets amplified DNA from Ostertagia lyrata, Haemonchus contortus, Trichostrongylus axei, Cooperia surnabada and Cooperia punctata. With respect to H. contortus, a closely migrating doublet was generated suggesting size heterogene- ity in the ETS which is consistent with multiple rDNA repeat units within this species. PCR analyses using mixtures of monospecifically-purified nematode eggs indicated a sensitivity of less than 0.5 egg-DNA equivalent per species. Although, not designed as a quantitative technique, relative PCR signal intensities corresponded to relative egg burdens within the DNA samples from mixed species of eggs. Published by Elsevier Science B.V

The Classification of Medical Events Using Latent Semantic Analysis

Clinical information is dominated by natural language representation of data and knowledge. To bring quantitative methods to bear in the empiric analysis of clinical episodes, they must be classified into reasonably homogenous categories that sustain inference and generalization. A tangible, if trivial, example of a classification requirement is the retrieval of patient cases relevant to the testing of a clinical hypothesis, so that they can be further scrutinized. Reliance on text word retrieval alone, drawn from natural language summaries, is fraught with contextual ambiguity and defeated by an expressively rich sub-language

Study of fuel cells using storable rocket propellants quarterly report no. 2, 18 may - 17 aug. 1965

Catalysts for Aerozine-50 reforming and nitrogen tetroxide decomposition for development of rocket fuel cells operating on storable propellan

Traumatic dissection and rupture of the abdominal aorta as a complication of the Heimlich maneuver

Although the Heimlich maneuver is considered the best intervention for relieving acute upper airway obstruction, several complications have been reported in the literature. These complications can occur as a result of an increase in abdominal pressure leading to a variety of well-documented visceral injuries, including the great vessels. Acute abdominal aortic thrombosis after the Heimlich maneuver is a rare but recognized event; however, to date no case of traumatic dissection and rupture of the abdominal aorta has been described. We report the first known case, to our knowledge, of a traumatic dissection and rupture of the abdominal aorta after a forcefully applied Heimlich maneuver

B633: The Production of Specific Pathogen Free Broilers in Maine

This bulletin describes the methods used and results obtained in the production of broiler chickens free of the common poultry diseases. It is hoped that this study may serve as a guide to those wishing to eradicate or control some of the common disease problems in broiler production.https://digitalcommons.library.umaine.edu/aes_bulletin/1037/thumbnail.jp

B584: Controlling Infectious Bronchitis in Maine Chickens

This publication reports on the investigations of some of the respiratory disease problems of domestic chickens and steps taken to improve the vaccines created to prevent the diseases.https://digitalcommons.library.umaine.edu/aes_bulletin/1030/thumbnail.jp

A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle

A multiplex polymerase chain reaction (PCR) test was developed for identifying gastrointestinal (GI) nematodes that commonly infect cattle. This assay was developed using adult-derived genomic DNA and shown capable of discriminating parasite eggs from the feces of experimentally-infected animals at both the species and genus levels. Sequence data from internal (ITS) and external (ETS) transcribed spacers of the ribosomal DNA (rDNA) repeats as well as the 3′-end of the small subunit rDNA and 5′-end of the large subunit rDNA were used to generate five primer sets which, when used simultaneously in a multiplex PCR, produce a unique electrophoretic DNA banding pattern charac- terized by a single DNA fragment for Ostertagia ostertagi (257 bp), Haemonchus placei (176 bp),Oesophagostomum radiatum (329 bp), Trichostrongylus colubriformis (243 bp) and Cooperia on- cophora (151 bp). In a similar manner, the constructed primer sets amplified DNA from Ostertagia lyrata, Haemonchus contortus, Trichostrongylus axei, Cooperia surnabada and Cooperia punctata. With respect to H. contortus, a closely migrating doublet was generated suggesting size heterogene- ity in the ETS which is consistent with multiple rDNA repeat units within this species. PCR analyses using mixtures of monospecifically-purified nematode eggs indicated a sensitivity of less than 0.5 egg-DNA equivalent per species. Although, not designed as a quantitative technique, relative PCR signal intensities corresponded to relative egg burdens within the DNA samples from mixed species of eggs. Published by Elsevier Science B.V

TB9: Newcastle Disease Virus Activity and Volume of Amniotic Allantoic Fluid in Chicken Embryos from Flocks with Different Vaccination Histories

Breeding hens of similar age, 9 to 11 months old, were selected from four flocks, each with a different vaccination history. One flock received no vaccinations, the second received spray and wing web Newcastle disease (ND) vaccine, the third was given three ND dust vaccinations, and the fourth received two dead ND viral vaccinations.https://digitalcommons.library.umaine.edu/aes_techbulletin/1191/thumbnail.jp

TB42: The Mycotoxic Effects of Fungi Isolated from Poultry Feed Ingredients: The Response of Ducklings and Performance of Commercial Broiler Chickens Fed Experimentally Infected Corn Diets

The present work, planned to investigate the possibility of mycotoxins occurring in feed ingredients fed to poultry in Maine, was designed with the following objectives: (1) to isolate fungi from poultry feed ingredients; (2) to grow the m separately on corn (the carbohydrate source of poultry rations) for later mixing into the diets; (3) to test the variously infected lots of this corn substrate for mycotoxicity by feeding ducklings, a bioindicator for toxins; (4) to determine the effects of aflatoxin and other mycotoxins from feed ingredients, on the performance of commercial broiler chickens; and (5) to appraise this response as a measure of toxicity of the fungi found in feed ingredients.https://digitalcommons.library.umaine.edu/aes_techbulletin/1159/thumbnail.jp